TABLE 1.
Primers for multiplex PCR amplification of Salmonella enterica serovars Typhi and Paratyphi A
| Gene and primer (oligonucleotide sequence) | Length (bp) | Amplified fragment size (bp) | Sourceb |
|---|---|---|---|
| tyv (rfbE) | |||
| tyv-s (5"-GAG GAA GGG AAA TGA AGC TTT T-3") | 22 | 615 | M29682 |
| tyv-as (5"-TAG CAA ACT GTC TCC CAC CAT AC-3") | 23 | M29682 | |
| prt (rfbS) | |||
| parat-s (5"-CTT GCT ATG GAA GAC ATA ACG AAC C-3") | 25 | 258 | M29682 |
| parat-as, (5"-CGT CTC CAT CAA AAG CTC CAT AGA-3") | 24 | M29682 | |
| viaB | |||
| vi-s (5"-GTT ATT TCA GCA TAA GGA G-3") | 19 | 439 | D14156 |
| vi-as (5"-CTT CCA TAC CAC TTT CCG-3") | 18 | D14156 | |
| fliC | |||
| fliCcom-s (5"-AAT CAA CAA CAA CCT GCA GCG-3") | 21 | L21912 | |
| fliCd-as (5"-GCA TAG CCA CCA TCA ATA ACC-3") | 21 | L21912 | |
| fliCa-as (5"-TAG TGC TTA ATG TAG CCG AAG G-3") | 22 | X03393 | |
| fliCcom-fliCd-as | 750 (489)a | ||
| fliCcom-fliCa-as | 329 |
a
Number in parentheses represents size of PCR product of H:j gene.
b
Primers were designed using sequences corresponding to indicated GenBank-EMBL-DDBL nucleotide sequence database accession numbers.