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Primers used for amplification of the pncA gene of M. tuberculosis. (A) Schematic presentation of the positions of the primers used. (B) Exact positions of the primers used. The sequence TGGT in primer ON00-0016 was converted to AGGA to give an optimum ribosome binding sequence (Shine-Dalgarno sequence) in primer ON00-0116. The sequence ATGC in primer ON00-0046 was converted to ATGA to give a Kozak consensus sequence in primer ON00-0036.
