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HCV grown in vivo has a lower buoyant density than HCV grown in vitro. The relative viral RNA levels (open circles) and buoyant density (filled circles) were plotted for each fraction. Gradients were loaded with HCVcc (A), plasma from chimpanzee 4x0483 at 3 weeks postinfection (B), and virus recovered from chimpanzee 4x0483 week 3, then passaged in cell culture for 9 days (C). Fraction 1 corresponds to the top of each gradient.
