Figure 4.

IL-1β slightly increases PAR-1 protein expression in the primary astrocyte cultures, but not that in neuronal nor mixed glial cultures. Mouse cortical neuronal, mixed glial and astrocyte cultures were treated with 5 ng/ml of rmIL-1β for 24 hr and 10 μg of protein was analyzed by Western blot. Increased ceruloplasmin expression demonstrated that the mixed glia and astrocytes responded to IL-1β. The blot was reprobed for β-tubulin to confirm equal protein loading. Data are representative of results obtained from three independent experiments.