Figure 2. Human fetal cardiac cells remove autologous apoptotic cells.

Cardiac cells were incubated with or without autologous apoptotic cells (5 hours or 18 hours, 37°C). (A) Cells were fixed, permeabilized, and stained using the TUNEL-peroxidase assay. (B) Confocal analysis of a single cell double-labeled with TUNEL-FITC (green) and anti–α-sarcomeric actinin (red) to identify the phagocytosing cell as a cardiocyte.