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. 2006 Aug 10;116(9):2413–2422. doi: 10.1172/JCI27803

Figure 6. Reactivity of affinity-purified human anti-Ro60 with wild-type and Ro60-knockout murine cells and effect on apoptotic uptake.

Figure 6

Splenocytes and cardiocytes were isolated from wild-type and Ro60-knockout C57BL/6 mice, as described in Methods. Splenocytes and cardiocytes were rendered apoptotic by plating on pHEMA plus TNF-α (10 ng/ml, 18 hours, 37°C). FACS analyses of digitonin-permeabilized nonapoptotic splenocytes, after incubation with affinity-purified anti-Ro60 antibodies, are shown in A (upper left: wild-type; lower left: Ro60-knockout). Results of FACS analyses of similarly incubated apoptotic cells are also shown (upper right: wild-type; lower right: Ro60-knockout). (B) Uptake of apopotic Ro60-knockout cardiocytes by neighboring healthy wild-type cardiocytes in the absence (N/A) and presence of: IgG from healthy human donor; affinity-purified anti-La48 (AP48); affinity-purified anti-Ro52; and affinity-purified anti-Ro60.