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. 2005 Oct 24;2005(5):263–272. doi: 10.1155/MI.2005.263

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Dependence of PAF formation by incubation time and protein concentration: (a) time course of PAF production using 0.01, 0.02, 0.04 mg total protein; (b) lyso-PAF AT activity as a function of protein concentration at a fixed incubation time 30 minutes. Experiments were performed with total membrane fractions of HMC in the present of 20 μM lyso-PAF and 200 μM acetyl-CoA. Results are the average of two independent determinations using different enzyme preparations performing duplicate samples

Dependence of PAF formation by incubation time and protein concentration: (a) time course of PAF production using 0.01, 0.02, 0.04 mg total protein; (b) lyso-PAF AT activity as a function of protein concentration at a fixed incubation time 30 minutes. Experiments were performed with total membrane fractions of HMC in the present of 20 μM lyso-PAF and 200 μM acetyl-CoA. Results are the average of two independent determinations using different enzyme preparations performing duplicate samples