Figure 4.

Effect of substrate concentration on lyso-PAF AT activity: (a) activity of lyso-PAF AT as a function of acetyl-CoA at fixed concentration of lyso-PAF (20 μM); (b) activity of lyso-PAF AT as a function of lyso-PAF at fixed concentration of acetyl-CoA (200 μM). Experiments were performed at total membrane fractions using 0.23 mg/mL protein in optimum conditions. Results represent the average ± SD from four independent determinations using different enzyme preparations performing duplicate samples