Figure 2.

Effect of nitric oxide donors on neutrophil apoptosis in vitro measured by DNA content. Neutrophils (1 × 10⁶ cells) were incubated at 37^°C with indicated stimuli (closed bars) or in the medium (open bars) for 8, 12, and 20 hours and apoptosis was determined as described in “materials and methods.” Data are presented as the means and SD percentages of cells with apoptotic nuclei. Values are statistically significant: neutrophils versus neutrophils with nitric oxide donors (∗ denotes that P = .003, ∗∗ denotes that P = .002).