Table 1.
Comparative analysis of apoptosis evaluated by ANX-FITC binding and cellular DNA content in the same sample of neutrophil culture. Neutrophils (1 × 106 cells) were incubated with the indicated stimuli for 8 and 12 hours and apoptosis was determined as described in “materials and methods.” Results are given as means and SD percentages of apoptotic cells for 6 separate experiments. Statistical significance: neutrophils versus neutrophils with NO donors (∗ denotes that P ≤ .05; ∗∗ denotes that P = .027).
| % of ANX-FITC+PI− and ANX-FITC+PI+ cells | % of cells with apoptotic nuclei | |||
| Agents | Time of neutrophils culture | Time of neutrophils culture | ||
| 8 h | 12 h | 8 h | 12 h | |
| Medium | 28 ± 8 | 57 ± 11 | 23 ± 7 | 49 ± 17 |
| GEA-3162 | 51 ± 1∗∗ | 73 ± 2∗ | 24 ± 4 | 61 ± 21∗∗ |
| Medium | 23 ± 8 | 48 ± 8 | 15 ± 6 | 17 ± 6 |
| SIN-1 | 62 ± 15∗∗ | 78 ± 5∗∗ | 24 ± 7∗ | 29 ± 13∗ |
| SNP | 23 ± 6 | 55 ± 12∗ | 15 ± 1 | 27 ± 8∗∗ |