Table 2.
Effect of exogenous superoxide dismutase (SOD) on apoptosis of neutrophils nontreated and treated by NO donors in vitro measured by ANX-FITC and PI binding. Neutrophils (1 × 106 cells) were incubated at 37° C with the indicated stimuli in the presence of SOD (300 U/mL) or not for 8 and 20 hours and apoptosis was determined as described in “materials and methods.” Results are given as means and SD percentages of the sum of cells binding ANX-FITC+PI− and ANX-FITC+PI+ for 6 separate experiments. Statistical significance: ∗∗ denotes neutrophils with SOD versus neutrophils with GEA-3162, SIN-1 (P ≤ .05); ∗ denotes neutrophils with GEA-3162+SOD and with SIN-1+SOD versus neutrophils with GEA-3162 and SIN-1, respectively (P ≤ .05). nd means no data.
| % of ANX-FITC+PI− and ANX-FITC+PI+ cells | ||
| Agents | Time of culture | |
| 8 h | 12 h | |
| SOD | 15 ± 3 | 42 ± 17 |
| GEA-3162 | 51 ± 9∗∗ | 90 ± 3∗∗ |
| GEA-3162+SOD | 38 ± 13∗ | 84 ± 3 |
| SIN-1 | 82 ± 13∗∗ | 88 ± 6∗∗ |
| SIN-1+SOD | 65 ± 10∗ | 84 ± 4 |
| SNP | 23 ± 6 | nd |
| SNP+SOD | 26 ± 7 | nd |