Table 3.
Effect of exogenous catalase (CAT) on apoptosis of neutrophils nontreated and treated by NO donors in vitro, measured by ANX-FITC and PI binding. Neutrophils (1 × 106 cells) were incubated with the indicated stimuli in the presence of CAT (1200 U/mL) or not for 8 and 20 hours and apoptosis was determined as described in “materials and methods.” Results are given as means and SD percentages of the sum of cells binding ANX-FITC+PI− and ANX-FITC+PI+ for 6 separate experiments. Statistical significance: ∗∗ denotes neutrophils with CAT versus neutrophils with NO donors, ∗ denotes neutrophils with SNP versus neutrophils with SNP+CAT (P ≤ .05).
| % of ANX-FITC+PI− and ANX-FITC+PI+ cells | ||
| Agents | Time of culture | |
| 8 h | 20 h | |
| CAT | 19 ± 5 | 33 ± 10∗∗ |
| GEA-3162 | 73 ± 7∗∗ | 79 ± 5∗∗ |
| GEA-3162+CAT | 71 ± 10 | 71 ± 13 |
| SIN-1 | 67 ± 10∗∗ | 76 ± 12∗∗ |
| SIN-1+CAT | 65 ± 8 | 70 ± 18 |
| SNP | 44 ± 16 | 57 ± 9 |
| SNP+CAT | 26 ± 7∗ | 40 ± 15∗ |