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. 2006 Aug;141(4):1237–1247. doi: 10.1104/pp.106.077693

Table II.

Kinetic parameters of the TaGlu1s and TaGlu1a mutants

The relative efficiency toward each substrate is the percent ratio of the k_cat/K_m values to that of TaGlu1a. n.d., Not detected. –, Not determined.

	DIBOA-Glc				DIMBOA-Glc				pNP-Glc
	K_m	k_cat	k_cat/K_m	Relative Efficiency	K_m	k_cat	k_cat/K_m	Relative Efficiency	K_m	k_cat	k_cat/K_m	Relative Efficiency
	mm	s⁻¹	s⁻¹/mm	%	mm	s⁻¹	s⁻¹/mm	%	mm	s⁻¹	s⁻¹/mm	%
TaGlu1a	1.40	48.8	34.6	100	0.36	338	939	100	1.85	99.2	53.6	100
TaGlu1b	1.44	214	149	429	0.29	1,201	4,138	441	2.16	128.2	59.3	111
TaGlu1c	1.05	137	131	377	0.39	773	1979	211	1.75	235.9	135	252
E191A	n.d.	n.d.	–	0	n.d.	n.d.	–	0	n.d.	n.d.	–	0
F198A	27.5	6.7	0.24	0.7	35.7	110	3.1	0.3	9.28	10.7	1.2	2.2
Y378A	6.05	26.4	4.36	12.6	1.19	124	104	11.4	1.33	25.1	18.9	35.3
Y378F	0.41	4.7	11.5	33.2	0.13	85.1	655	69.8	1.23	46.1	37.5	70.0
E407A	n.d.	n.d.	–	0	n.d.	n.d.	–	0	n.d.	n.d.	–	0
S464F	2.89	84.0	29.1	84.1	1.42	73.3	51.6	5.5	2.31	31.2	13.5	25.2
F471Y	4.21	83.5	19.8	57.2	0.70	192	274	29.2	2.93	54.6	18.6	34.7