Figure 10.

Effects of MG132 treatment on the cytoplasmic streaming. A, Control tube showing particles which moved quickly toward the tip in the tube center, and then moved away from the tip relatively slowly along the cell cortex (indicated by arrows; see also Supplemental Video 1). B, In the tube strongly inhibited by MG132, cytoplasmic streaming was almost completely stopped, especially in the apex and subapical region. (indicated by arrows; Supplemental Video 3). C, In the tubes treated with 40 μm MG132 for 20 h, and subsequently recovered in fresh culture medium for 1 h, vacuoles gradually disappeared from the tip (indicated by arrows) and accelerated cytoplasmic streaming was resumed (Supplemental Video 4). Bar = 20 μm.