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Proteasome activity measurement. Extracts of 24 h cultured pollen tubes were incubated in the presence of 120 μm sLLVY-AMC and various concentrations of MG132 at 24°C. The breakdown of the fluorogenic peptide was monitored by a fluorescence spectrophotometer for 5 min. MG10, MG20, MG40, and MG80 represent additions of 10, 20, 40, or 80 μm MG132, respectively, into extracts.
