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. 2006 Aug;141(4):1578–1590. doi: 10.1104/pp.106.081703

Figure 4.

Figure 4.

SDS-PAGE and immunoblotting analysis. Lanes M6, 12, 18, and 24 represent pollen grains/tubes treated with 40 μm MG132 for 6, 12, 18, and 24 h, respectively; lanes C6, 12, 18, and 24 represent pollen grains/tubes cultured under control conditions for 6, 12, 18, and 24 h, respectively. Molecular masses (in kilodaltons) of standard proteins are indicated on the left. A, Protein pattern obtained by SDS-PAGE and Coomassie Blue staining. B, Immunoblotting analysis with antiubiquitin antibody. C, Immunoblotting analysis with an antiactin antibody.