Figure 3.

Kn1-N2;Xcl1 seedling phenotypes. A, The number of knots on seedling leaves is reduced when Xcl1 is present. B, Semiquantitative RT-PCR of KN1 expression reveals that KN1 RNA is present in double mutant leaves with suppressed knots. Numbers in brackets indicate number of PCR cycles. Control RT-PCR for the constitutively expressed ZmGAPDH. C, GA20 oxidase levels are increased when Xcl1 is present and decreased in Kn1-N2 single mutants. D, Knots per leaf on the third leaf of Kn1-N2 heterozygote seedlings treated with Tween 20 (control), Uniconazole, or GA. Differences between Uniconazole and GA treatments were found to be statistically significant at P = 0.01 and differences between control and GA at P = 0.05 using ANOVA. Error bars are ses, n = 3. E, Detection of GA2 oxidase RNA levels with QRT-PCR in wild-type (wt), Xcl1, Kn1-N2 (Kn1), and Xcl1/Kn1-N2 seedlings. The data represents two replicate runs. RNA levels were normalized to ZmGAPDH mRNA and are shown relative to wild-type levels. Bars represent ses over four technical replicates.