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. 2002 Feb;40(2):524–531. doi: 10.1128/JCM.40.2.524-531.2002

FIG. 1.

FIG. 1.

Enzyme profiles of VP7 segments digested with three restriction enzymes, RsaI, AluI, and EcoRV. All products were analyzed by agarose gel (2%) electrophoresis and visualized by staining with ethidium bromide (0.1 mg/ml). (a) RsaI digestion. Lanes M, molecular size marker, 100-bp ladder; lane 1, undigested VP7 fragment; lane 2, DNA control digested with RsaI; lane 3, CIT-54RV (G1); lane 4, CIT-124RV (G4); lane 5, CIT-179RV (G1); lane 6, CIT-202RV (G4); lane 7, CIT-222RV (G4); lane 8, CIT-70RV (G1); lane 9, CIT-76RV (G1); lane 10, CIT-78RV (G1); lane 11, CIT-79RV (G1); lane 12, CIT-95RV (G2); lane 13, CIT-155RV (G1 + G4); lane 14, CIT-109RV (G1); lane 15, CIT-112RV (G1); lane 16, CIT-313RV (G1). (b) AluI digestion. Lanes M, molecular size marker, 100-bp ladder, and molecular size marker, grade V; lane 1, undigested VP7 fragment; lane 2, DNA control digested with AluI; lane 3, CIT-68RV (G2); lane 4, CIT-245RV (G2); lane 5, CIT-128RV (G2); lane 6, CIT-152RV (G2); lane 7, CIT-123RV (G2); lane 8, CIT-132RV (G1); lane 9, CIT-176RV (G4); lane 10, CIT-178RV (G4); lane 11, CIT-224RV (G4); lane 12, CIT-273RV (G2); lane 13, CIT-278RV (G4); lane 14, CIT-289RV (G4); lane 15, CIT-293RV (G4); lane 16, CIT-298RV (G4); lane