Table I.
Enhanced expression of ABA-inducible genes in PP2C mutants with respect to wild type
Numbers indicate the induction level of the stress-responsive genes under mock or ABA treatment (10 μm for 3 h) in wild type and mutants. Values are the expression level reached in each mutant genotype with respect to the wild type (value 1). qRT-PCR analyses were made in triplicate on RNA samples obtained from mock-treated plants or plants treated once with 10 μm ABA.
| Genotype
|
||||||
|---|---|---|---|---|---|---|
| RAB18 | KIN1 | RD22 | P5CS1 | RD29a | RD29b | |
| Mock | ||||||
| Col | 1 | 1 | 1 | 1 | 1 | 1 |
| hab1-1 | 0.9 | 0.8 | 1.5 | 0.9 | 2.7 | 2.0 |
| abi1-2 | 1.1 | 1.0 | 1.4 | 1.1 | 1.3 | 2.6 |
| abi1-3 | 0.8 | 0.7 | 1.1 | 0.8 | 1.0 | 2.6 |
| hab1-1 abi1-2 | 2.1 | 1.6 | 2.1 | 1.7 | 2.0 | 2.5 |
| hab1-1 abi1-3 | 2.3 | 1.2 | 1.6 | 1.9 | 2.0 | 2.7 |
| ABA treatment | ||||||
| Col | 1 | 1 | 1 | 1 | 1 | 1 |
| hab1-1 | 2.6 | 1.7 | 2.5 | 3.0 | 3.5 | 2.1 |
| abi1-2 | 3.7 | 1.6 | 1.7 | 2.6 | 1.7 | 2.4 |
| abi1-3 | 2.7 | 1.5 | 1.5 | 2.0 | 1.5 | 1.3 |
| hab1-1 abi1-2 | 6.0 | 2.7 | 3.0 | 6.4 | 2.2 | 3.9 |
| hab1-1 abi1-3 | 8.6 | 3.6 | 3.1 | 6.0 | 2.2 | 4.9 |