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Expression of YSL1 and YSL3 is metal regulated. Col-0 plants were grown on complete MS for 7 d, then transferred to MS − Cu, Mn, or Zn for 7 d, or to MS − Fe for 3, 5, or 7 d. A, RT-PCR using total RNA from shoots. Amplification of YSL1, YSL3, or 18S rRNA is shown. B, RT-PCR using total RNA from roots. Amplification of YSL1, YSL3, or 18S rRNA is shown. As a positive control for Fe deficiency, IRT1 was also amplified.
