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. 2006 Aug;141(4):1446–1458. doi: 10.1104/pp.106.082586

Figure 7.

Figure 7.

A, Semiquantitative RT-PCR of YSL1 and YSL3 in senescing leaves. Detached leaves were incubated on deionized water in either light or darkness to induce senescence. B to E, Histochemical staining of YSL1 and YSL3 promoter-GUS reporter plants during leaf senescence. Rosette leaf number 6 (the sixth true leaf to emerge) was stained for histochemical detection of GUS activity for 6 to 9 h. B, Leaf of a YSL1-GUS plant at day 20. C, Leaf of a YSL1-GUS plant at day 38. D, Leaf of a YSL3-GUS plant at day 20. E, Leaf of a YSL3-GUS plant at day 38. F, Change in leaf metal concentration during senescence. The distal half of leaves 5 and 6 of Col-0 and ysl1ysl3 plants was collected at 28 and 38 d after sowing and ions were measured by inductively coupled plasma mass spectrometry. Values are expressed as the percent difference in metal concentration from day 28 to day 38. t test of the percent change arrays indicates no significant difference between the two genotypes for Mn, Fe, Co, or Mo, but P < 0.05 for Zn and P < 0.01 for P and Cu.