Figure 3.

Methylation interference experiments of RISBZ1 and RPBF binding to NRP33 promoter. DNA fragments corresponding to NRP33 (−386 to −276 and −295 to −172) were labeled on both strands (top and bottom). Both strands were partially methylated and incubated with GST-RISBZ1 or GST-RPBF. Free (F) and retarded (S) protein-DNA complex fragments were separated by PAGE. Fragments were eluted from the gel, chemically digested with piperidine, and separated on a sequencing gel in parallel with the sequencing ladder of this fragment.