Figure 3.

Expression of AtPAO1 in E. coli. Total cellular extracts (T), total soluble proteins (S), and inclusion bodies (B) from E. coli transformed with the AtPAO1-pET17b construct have been analyzed by immunoblotting using an anti-6-His-tag antibody. Expression of recombinant protein in E. coli has been induced by 0.4 mm IPTG at 24°C for 5 h (I). A part of the same culture was allowed to grow for the same time period in the absence of IPTG (NI) as a control. The various fractions have been normalized for the volume of the initial culture. MPAO, Recombinant MPAO expressed in P. pastoris. A representative experiment, which has been repeated twice, is shown.