Table II.

Inhibition constants of recombinant AtPA01 by various plant and animal PAO inhibitors

Compound	Kia
	μm
Guazatine	0.7 ± 0.4
N-Prenylagmatine	4.0 ± 0.5
1,12-Diaminododecane	11.7 ± 0.8
MDL72527	137.3 ± 32.7
Agmatine	147.0 ± 8.2
1,8-Diaminooctane	293.0 ± 30.5
Spermidine	546.0 ± 40.0

^aK_i values were determined using Spm as substrate in 100 mm Tris-HCl, pH 8.0, for all inhibitors except in the case of 1,12-diaminododecane, for which the K_i value was determined in 100 mm Tris-HCl, pH 7.0. The K_m for Spm in 100 mm Tris-HCl, pH 7.0, was 200 μm. Data are mean ± se of three independent experiments.