Molecular analysis of 35S:FsPP2C2 transgenic lines. A, RNA-blot analysis of transgenic lines overexpressing FsPP2C2. Total RNA (10 μg/line) from wild-type plants and C1 to C3 transgenic plants was isolated and hybridized with a specific FsPP2C2 probe. Bottom, Ethidium bromide-stained gel showing rRNAs. B, Southern-blot analysis of wild-type (WT; Col-0 background) and transgenic lines (C1–C3) overexpressing FsPP2C2. Genomic DNA was digested with HindIII, blotted onto a nylon membrane, and hybridized with a FsPP2C2-specific probe.