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. 2006 Aug;18(8):1961–1974. doi: 10.1105/tpc.106.041269

Figure 1.

Figure 1.

Suppression of Oleosins in Arabidopsis.

(A) Scale diagram of the constructs used to suppress OLEO1 using RNAi methods. The Antisense, Hairpin, and Loop cassettes are shown.

(B) Scale diagram of the insertion of a T-DNA element into OLEO1 and OLEO2 genes (lines KnockOLEO1 and KnockOLEO2, respectively). Each gene has two exons (thick line) and one intron (thin line). Both T-DNA insertions are located in the middle of the second exon.

(C) SDS-PAGE profile of oilbody-associated proteins in different plants. The first lane (L) contains the protein ladder (Benchmark; Invitrogen). The second lane contains the oilbody-associated proteins from wild-type (C24) plants. Lanes numbered 1 to 3 contain the oilbody-associated proteins from SupOLEO1-Loop, SupOLEO1-Hairpin, and SupOLEO1-Antisense plants, respectively. Lanes 4 and 5 contain the oilbody-associated proteins from KnockOLEO1 and KnockOLEO2 lines, respectively. The positions of the three most abundant oleosin isoforms are indicated by arrows.