Figure 2.

Expression Analysis of P. hybrida B- and C-Class MADS Box Genes in Floral Whorls of Wild-Type and Various Mutant Flowers as Determined by Real-Time PCR.

Organ types are indicated above bars showing gene expression levels as follows: se, sepal; pe, petal; st, stamen; ca, carpel. Homeotically converted organs are shown underlined. All reactions were performed in duplicate using a biological replicate for each sample (represented by pairs of gray and black bars). The height of the bars for a given gene indicates relative differences in expression levels within the range of tissues tested. For each primer combination separately, the highest expression value encountered in the series of different tissues tested was set equal to 100, and lower values are plotted relative to this highest value on a linear y axis scale. GAPDH (for glyceraldehyde-3-phosphate dehydrogenase) expression levels were used for normalization.