Table 1.
Analysis of Elongation, Dehiscence, and ARF8 Expression Ratios in Lines Used in This Study
| Line | Silique Lengtha | Elongationb | Dehiscenceb | mRNA Expression Ratioc |
|---|---|---|---|---|
| Ler | 4.5 mm ± 0.5 mm | − | − | 1.0 |
| arf8-4 | 7.5 mm ± 0.8 mm | +++ | ++ | 1.6 ± 0.2 |
| Col | 3.7 mm ± 0.5 mm | − | − | 1.0 |
| arf8-4NIL | 5.9 mm ± 0.7 mm | ++ | ++ | 1.3 ± 0.1 |
| arf8-5 | 4.0 mm ± 0.6 mm | + | − | 2.4 ± 0.2 |
| arf8-6 | 5.2 mm ± 0.2 mm | ++ | ++ | 0.6 ± 0.2 |
| Ws | 3.8 mm ± 0.3 mm | − | − | 1.0 |
| arf8-1 | 5.0 mm ± 0.1 mm | ++ | ++ | 0.2 ± 0.1 |
| arf8-4 + ARF8 #1 | 5.4 mm ± 0.8 mm | + | ++ | 4.1 ± 1.2 |
| arf8-4 + ARF8 #2 | 5.1 mm ± 0.6 mm | + | ++ | 1.0 ± 0.1 |
| arf8-4 + ARF8 #3 | 4.3 mm ± 0.7 mm | − | ++ | ND |
| arf8-4 + ARF8 #4 | 5.6 mm ± 0.6 mm | ++ | ++ | 1.2 ± 0.2 |
| arf8-4 + ARF8 #5 | 5.0 mm ± 0.7 mm | + | ++ | 3.0 ± 0.2 |
| arf8-4 + ARF8 #6 | 4.9 mm ± 0.6 mm | + | ++ | ND |
| arf8-4 + ARF8 #7 | 5.4 mm ± 0.6 mm | + | ++ | 5.9 ± 0.3 |
| arf8-4 + ARF8 #8 | 5.7 mm ± 0.5 mm | ++ | ++ | ND |
| arf8-4 + ARF8 #9 | 6.0 mm ± 0.8 mm | ++ | ++ | ND |
| Ler + ARF8 #1 | 3.8 mm ± 0.7 mm | − | − | ND |
| Ler + ARF8 #2 | 4.3 mm ± 0.4 mm | − | − | ND |
| Ler + ARF8 #3 | 4.6 mm ± 0.4 mm | − | − | ND |
a
Pistil lengths measured 7 d after emasculation (±sd; a minimum of 40 flowers was emasculated and measured for each line).
b
−, no elongation/dehiscence; +, some elongation; +++/++, very good/good elongation/dehiscence.
c
The expression ratio of ARF8 mRNA was determined from flowers at anthesis. The ratio for each line is determined in relation to the expression in the corresponding wild-type line, which was set to 1.0 as the reference point. ND, not determined.