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Two-dimensional phosphotryptic mapping of pRB derived from wild-type or p21−/− irradiated cells. pRB was immunoprecipitated from either wild-type or p21−/− irradiated cells and subjected to trypsin digestion. The resulting phosphopeptides were resolved by electrophoresis and ascending chromatography and visualized by autoradiography. ∗ denotes phosphopeptides that were present in maps from Rb−/− cells and therefore are not derived from pRB.
