FIG. 1.
PCR assay of peripheral blood from dogs during acute CME. Three dogs were tested with the nested p30-based PCR described in the text. Buffy coat DNA preparations were assayed from two dogs prior to (lanes 1 and 2) and 21 days after (lanes 3 and 4) inoculation with E. canis Ebony isolate-infected blood from an experimentally infected dog (lane 5). The molecular size standard (M) is a 100-bp ladder.