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Specificity of the p30-based nested PCR assay. Lane 1 is the template-free control, lanes 2 and 3 represent amplification of 50 ng of E. canis Ebony and Oklahoma isolates, respectively, and lanes 4 to 6 represent assays of 50 ng of DNA isolated from E. chaffeensis, HGE agent, and E. muris, respectively. The 100-bp ladder (M) serves as the molecular size standard.
