TABLE 1.
Oligonucleotide sequences of E. canis p30 primer sets
| Primer set | Primer (sequence)
|
|
|---|---|---|
| Forward | Reverse | |
| 1 | ECA30-384S (ATAAACACGCTGACTTTACTGTTCC) | ECA30-583A (GTGATGAGATAGAGCGCAGTACC) |
| 2 | ECA30-387S (AACACGCTGACTTTACTGTTCC) | ECA30-597A (ATGGCTGCCGATGTGTGATG) |
| 3 | ECA30-390S (ACGCTGACTTTACTGTTCCAAAC) | ECA30-597A |
| 4 | ECA30-351S (AACATGATTGGGATGGAAGTC) | ECA30-591A (GCCGATGTGTGATGAGATAG) |
| 5 | ECA30-350S (AAACATGATTGGGATGGAAGTC) | ECA30-591bA (GCCGATGTGTGATGAGATAGAG) |
| 6 | ECA30-356S (GATTGGGATGGAAGTCCAATAC) | ECA30-591A |
| 7 | ECA30-356S | ECA30-591bA |
| 8 | ECA30-388S (ACACGCTGACTTTACTGTTCCAAAC) | ECA30-597bA (ATGGCTGCCGATGTGTGATGAG) |
| 9 | ECA30-282S (GTGTCTCACATTTTGGTAGCTTCTC) | ECA30-486A (CTTGGGCCACCCATTGAGTAAC) |
| 10 | ECA30-356S | ECA30-589A (CGATGTGTGATGAGATAGAGC) |
| 11 | ECA30-355S (TGATTGGGATGGAAGTCCAATAC) | ECA30-589bA (CGATGTGTGATGAGATAGAGCG) |
| 12 | ECA30-353S (CATGATTGGGATGGAAGTCCAATAC) | ECA30-597A |
| 13 | ECA30-278S (CCAAGTGTCTCACATTTTGGTAGC) | ECA30-484A (TGGGCCACCCATTGAGTAAC) |