Figure 5.

SiRNA-mediated depletion of clathrin impairs Wnt3A-stimulated reporter gene expression. LSL cells were transfected in two rounds with either empty plasmid vector (Mock) or siRNA corresponding to clathrin heavy chain (siCHC). (a) Levels of clathrin heavy chain and GSK3β were assessed by immunoblotting. (b) Cells were stimulated with purified Wnt-3A (~100 ng/mL) for approximately 8 hours, followed by lysis of cells and measurement of TOPFLASH and LacZ activities. Values represent means ± SEM from a representative experiment from 8 independent determinations.