Table 1.
RNA c/ml | TU/ml | pg p24/ml | TU/pg | TU/RNA | |
Cell factories | |||||
CH-eGFP-WS | 2.68 ± 0.38 × 1010 | 2.23 ± 1.10 × 109 | 9.8 ± 5.3 × 106 | 228 | 0.0832 |
Culture dishes | |||||
H-eGFP | 5.63 ± 0.50 × 1010 | 5.05 ± 4.9 × 107 | 4.67 ± 4.5 × 106 | 11 | 0.0009 |
H-eGFP-WS | 3.83 ± 2.25 × 1010 | 2.92 ± 2.5 × 108 | 4.83 ± 4.70 × 106 | 60 | 0.0076 |
CH-eGFP-WS | 2.73 ± 1.59 × 1010 | 1.68 ± 1.3 × 109 | 4.79 ± 3.45 × 106 | 351 | 0.0615 |
The lentiviral vector CH-eGFP-WS was produced in cell factories and concentrated by centrifugation as described before [8]. Three independent RNA extractions were carried out on this vector and RNA equivalents were determined by RT-qPCR. Mean values ± standard deviation are shown. Next, three lentiviral vectors with different transfer plasmids, H-eGFP, H-eGFP-WS and CH-eGFP-WS, were produced in parallel in cell culture dishes. RNA equivalents (RNA/ml), transducing units (TU/ml) and p24 concentrations (pg p24/ml) were determined by RT-qPCR, titration and ELISA, respectively. The TU/pg and TU/RNA value indicate the specific activity of the vector constructs and correlate well with the vector backbones. The data represent the mean values ± standard deviation of three independent productions per lentiviral vector.