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Sensitivity limits of the ompA VD4 real-time PCR assay. Tenfold dilutions of DNA extracted from C. pneumoniae isolate ATCC VR1360 and with a known titer (IFU per milliliter) were used in the assay. The number of IFU per dilution, fluorescence measurement, and data analysis were determined as described in Materials and Methods. The limit of detection of the real-time PCR assay was 0.001 IFU per PCR. ΔRn, fluorescence emission values. Open square, negative control.
