TABLE 3.
PCR assays for C. pneumoniae detection
| Assay format | Target | Primer or probe designation | Primer or probe sequence (5" → 3") | Amplicon size (bp) | Taq DNA polymerase | DNA/PCR (μl) | Source or reference |
|---|---|---|---|---|---|---|---|
| Real-time VD2 | ompA | VD2F | CGT TGG TTT ATT CGG AGT TA | 108 | Hot start | 5/25 | This study |
| VD2R | CCA AGA GAA AGA GGT GTC TGT | ||||||
| VD2 probe | FAM-TGT AAA TGC AAA TGA ACT ACC AAA CGT TTC-TAMRA | ||||||
| Real-time VD4 | ompA | VD4F | TCC GCA TTG CTC AGC C | 125 | Hot start | 5/25 | This study |
| VD4R | AAA CAA TTT GCA TGA AGT CTG AGA A | ||||||
| VD4 probe | FAM-TAA ACT TAA CTG CAT GGA ACC CTT CTT TAC TAG G-TAMRA | ||||||
| Nested touchdown | ompA | CP1 | TTA CAA GCC TTG CCT GTA GG | 333 | Regular | 10/50 | 45 |
| CP2 | GCG ATC CCA AAT GTT TAA GGC | ||||||
| CPC | TTA TTA ATT GAT GGT ACA ATA | 207 | |||||
| CPD | ATC TAC GGC AGT AGT ATA GTT | ||||||
| Nested | 16S rRNA | OF | ACG GAA TAA TGA CTT CGG | 436 | Hot start | 10/50 | 32 (Modified) |
| OR | TAC CTG GTA CGC TCA AAT | ||||||
| IF | CGG AAT AAT GAC TTC GGT TGT TAT TTA G | 224 | |||||
| IR | TCA TCG CCT TGG TGG GCT T |