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. Author manuscript; available in PMC: 2006 Aug 9.
Published in final edited form as: J Biol Chem. 2000 Jun 30;275(26):19620–19627. doi: 10.1074/jbc.M001333200

Fig. 4. Concentration-dependent stimulation of BiP ATPase activity by J-MTJ1.

Fig. 4

A, stimulation of His10-BiP steady-state ATPase activity by increasing concentrations of wild-type J-MTJ1 and mutant J-MTJ1:H89Q. His10-BiP (0.2 μm) was incubated with increasing concentrations (0.03–10 μm) of wild-type J-MTJ1 (closed circles) or mutant J-MTJ1:H89Q (open circles) and assayed for ATPase activity as described under “Experimental Procedures.” B, double reciprocal plot of BiP ATPase activity stimulated by J-MTJ1.