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. Author manuscript; available in PMC: 2006 Aug 9.
Published in final edited form as: J Biol Chem. 2000 Jun 30;275(26):19620–19627. doi: 10.1074/jbc.M001333200

Fig. 5. Complex formation between BiP and J-MTJ1.

Fig. 5

Interaction of His6-BiP with J-MTJ1 (left panels) or J-MTJ1: H89Q (right panels) was assayed as described under “Experimental Procedures.” Top panels, Coomassie-stained 15% SDS-PAGE. Lane 1, His6-BiP; lane 2; cleaved J-MTJ1 (or, right panel, mutant J-MTJ1:H89Q); lane 3, His6-BiP with wild-type J-MTJ1 (or, right panel, mutant J-MTJ1:H89Q); lane 4, His6-BiP with wild-type (or, right panel, mutant J-MTJ1:H89Q) in the presence of 1 mm ATP; lane 5, His6-BiP with wild-type (or, right panel, mutant J-MTJ1:H89Q) in the presence of 1 mm ADP. Bottom panel, densitometry scanning of the SDS-PAGE. The y axis represents the average of three independent experiments.