Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1995 Jun;100(3):514–518. doi: 10.1111/j.1365-2249.1995.tb03731.x

Composition and biological behaviour of immune complexes isolated from synovial fluid of patients with juvenile rheumatoid arthritis (JRA).

J N Jarvis 1, M M Diebold 1, M K Chadwell 1, M Iobidze 1, H T Moore 1
PMCID: PMC1534475  PMID: 7774064

Abstract

Data published from in vitro studies have shown that IgM-rheumatoid factor (RF)-bearing immune complexes possess several biological features that may contribute to their pathogenicity. However, no studies have demonstrated that such complexes exist at sites of inflammation in children with rheumatoid disease. We used two methods of sequential column chromatography to purify immune complexes from synovial fluids of children with JRA. We demonstrate that high molecular weight complexes contain IgM-RF, have not bound C4 in vivo, but activate the classical pathway in vitro. In contrast, complexes which have bound C3 in vivo do not contain IgM-RF and are weak complement activators in vitro.

Full text

PDF

Images in this article

516Fig. 1
on p.516
516Fig. 2
on p.516

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cassidy J. T., Levinson J. E., Bass J. C., Baum J., Brewer E. J., Jr, Fink C. W., Hanson V., Jacobs J. C., Masi A. T., Schaller J. G. A study of classification criteria for a diagnosis of juvenile rheumatoid arthritis. Arthritis Rheum. 1986 Feb;29(2):274–281. doi: 10.1002/art.1780290216. [DOI] [PubMed] [Google Scholar]
  2. Jarvis J. N., Iobidze M., Taylor H., DeJonge J., Chang S. A comparison of immunoglobulin G-containing high-molecular-weight complexes isolated from children with juvenile rheumatoid arthritis and congenital human immunodeficiency virus infection. Pediatr Res. 1993 Dec;34(6):781–784. doi: 10.1203/00006450-199312000-00017. [DOI] [PubMed] [Google Scholar]
  3. Jarvis J. N., Lockman J. C., Levine R. P. IgM rheumatoid factor and the inhibition of covalent binding of C4b to IgG in immune complexes. Clin Exp Rheumatol. 1993 Mar-Apr;11(2):135–141. [PubMed] [Google Scholar]
  4. Jarvis J. N., Pousak T., Krenz M. Detection of IgM rheumatoid factors by enzyme-linked immunosorbent assay in children with juvenile rheumatoid arthritis: correlation with articular disease and laboratory abnormalities. Pediatrics. 1992 Dec;90(6):945–949. [PubMed] [Google Scholar]
  5. Jarvis J. N., Pousak T., Krenz M., Iobidze M., Taylor H. Complement activation and immune complexes in juvenile rheumatoid arthritis. J Rheumatol. 1993 Jan;20(1):114–117. [PubMed] [Google Scholar]
  6. Jarvis J. N., Taylor H., Iobidze M., Krenz M. Complement activation and immune complexes in children with polyarticular juvenile rheumatoid arthritis: a longitudinal study. J Rheumatol. 1994 Jun;21(6):1124–1127. [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Latzka U., Menzel E. J., Granninger W., Steffen C. Immune complexes in juvenile chronic arthritis. Rheumatol Int. 1984;4(1):45–48. doi: 10.1007/BF00683885. [DOI] [PubMed] [Google Scholar]
  10. Moore T. L., Dorner R. W., Osborn T. G., Zuckner J. Hidden 19S IgM rheumatoid factors. Semin Arthritis Rheum. 1988 Aug;18(1):72–75. doi: 10.1016/0049-0172(88)90036-4. [DOI] [PubMed] [Google Scholar]
  11. Moore T. L., Dorner R. W., Sheridan P. W., Weiss T. D., Baldassare A. R., Osborn T. G., Zuckner J. Longitudinal study of the presence of hidden 19S IgM rheumatoid factor in juvenile rheumatoid arthritis. J Rheumatol. 1982 Jul-Aug;9(4):599–602. [PubMed] [Google Scholar]
  12. Moore T. L., Sheridan P. W., Traycoff R. B., Zuckner J., Dorner R. W. Immune complexes in juvenile rheumatoid arthritis: a comparison of four methods. J Rheumatol. 1982 May-Jun;9(3):395–401. [PubMed] [Google Scholar]
  13. Ng Y. C., Peters D. K., Walport M. J. Monoclonal rheumatoid factor-IgG immune complexes. Poor fixation of opsonic C4 and C3 despite efficient complement activation. Arthritis Rheum. 1988 Jan;31(1):99–107. doi: 10.1002/art.1780310114. [DOI] [PubMed] [Google Scholar]
  14. O'Sullivan M. M., Amos N., Williams B. D. Complement mediated inhibition of immune precipitation in rheumatoid arthritis: studies on interaction of heat aggregated IgG with IgM rheumatoid factor. Ann Rheum Dis. 1988 Aug;47(8):675–680. doi: 10.1136/ard.47.8.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Olsen N. J., Stastny P., Jasin H. E. High levels of in vitro IgM rheumatoid factor synthesis correlate with HLA-DR4 in normal individuals. Arthritis Rheum. 1987 Aug;30(8):841–848. doi: 10.1002/art.1780300801. [DOI] [PubMed] [Google Scholar]
  16. Ploski R., Vinje O., Rønningen K. S., Spurkland A., Sørskaar D., Vartdal F., Førre O. HLA class II alleles and heterogeneity of juvenile rheumatoid arthritis. DRB1*0101 may define a novel subset of the disease. Arthritis Rheum. 1993 Apr;36(4):465–472. doi: 10.1002/art.1780360406. [DOI] [PubMed] [Google Scholar]
  17. Schifferli J. A., Ng Y. C., Peters D. K. The role of complement and its receptor in the elimination of immune complexes. N Engl J Med. 1986 Aug 21;315(8):488–495. doi: 10.1056/NEJM198608213150805. [DOI] [PubMed] [Google Scholar]
  18. Thomsen B. S., Heilmann C., Jacobsen S. E., Pedersen F. K., Morling N., Jakobsen B. K., Svejgaard A., Nielsen H. Complement C3b receptors on erythrocytes in patients with juvenile rheumatoid arthritis. Arthritis Rheum. 1987 Sep;30(9):967–971. doi: 10.1002/art.1780300902. [DOI] [PubMed] [Google Scholar]
  19. Vaughan J. H. 1992 Joseph J. Bunim Lecture. Pathogenetic concepts and origins of rheumatoid factor in rheumatoid arthritis. Arthritis Rheum. 1993 Jan;36(1):1–6. doi: 10.1002/art.1780360102. [DOI] [PubMed] [Google Scholar]
  20. Walport M. J., Lachmann P. J. Erythrocyte complement receptor type 1, immune complexes, and the rheumatic diseases. Arthritis Rheum. 1988 Feb;31(2):153–158. doi: 10.1002/art.1780310201. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES