Figure 5.

Adjuvant effects of FL and GM-CSF in ovalbumin-immunized mice. Shown are antigen-specific, secondary serum antibody titers in FL- and GM-CSF-treated mice after challenge with ovalbumin. PBS- or cytokine-treated mice were primed and boosted with ovalbumin, and secondary anti-ovalbumin antibody responses were measured by ELISA. Each dot represents a single mouse. Data are pooled from three independent experiments, each of which had five mice per group. Because the data are not normally distributed, the Wilcoxon rank sum 2-sided test has been used to analyze the P values. P values comparing the differences between the PBS, GM-CSF, and FL groups are shown above each graph.