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Interactions of SUT2 and SUC2 as baits with other sucrose transporters detected by the split-ubiquitin system using LacZ as a reporter gene. Interaction of SUT2-CubPLV and SUC2-CubPLV as a bait was tested against various NubG-fusions of membrane proteins and soluble proteins as prey. Positive interaction was visualized by β-galactosidase expression in filter assays.
