Abstract
The level of serum IgM, IgG and IgA antibodies including IgG1, IgG2, IgG3, IgG4, IgA1 and IgA2 subclass-specific antibodies to Bacteroides (Porphyromonas) gingivalis fimbriae and to lipopolysaccharide (LPS) were analysed in patients with different forms of periodontal disease (PD) and control subjects by ELISA. Among PD subjects, sera obtained from adult periodontitis (AP), rapidly progressive periodontitis (RPP) and gingivitis contained high titres of fimbriae-specific IgG antibodies (7500-15,000 ELISA units) followed by IgA (90-700 units) and IgM (30-90 units). In contrast, sera from localized juvenile periodontitis (LJP) subjects exhibited much lower titres of fimbriae-specific IgG (89 +/- 11 units), IgA (31 +/- 5 units) and IgM (17 +/- 3 units) antibodies. A similar response pattern was also seen in sera from normal subjects aged 35-41 years who practice normal oral hygiene, while sera of younger adults (aged 18-24) with superior hygiene did not have any antigen-specific antibodies. Analysis of IgG subclass anti-fimbriae responses revealed that the major response was IgG3 followed by IgG1, IgG2 and IgG4 in AP, RPP and gingivitis. Although lower, a similar pattern of IgG subclass titre was seen in LJP and normal subjects aged 35-41 years. When IgA subclass responses were measured in AP and RPP, higher titres of the fimbriae-specific response were noted with IgA1 when compared with IgA2. However, lower but approximately equal levels of fimbriae-specific IgA1 and IgA2 titres were seen in other PD groups. When anti-B. gingivalis LPS-specific responses were measured, the sera of AP patients contained high levels of IgG antibodies (2265 +/- 224 units) followed by IgA (411 +/- 90 units) and IgM (214 +/- 56 units). Further, IgG anti-LPS responses were mainly IgG2 followed by IgG4, IgG3 and IgG1. For IgA subclass responses, higher titres of anti-LPS-specific antibodies were noted in IgA2 subclass over IgA1. These results showed that higher anti-B. gingivalis antibody responses occur in PD when compared with healthy individuals and protein and lipid-carbohydrate antigens of B. gingivalis induce distinct patterns of antigen-specific IgG and IgA subclass responses.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aalberse R. C., van der Gaag R., van Leeuwen J. Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response. J Immunol. 1983 Feb;130(2):722–726. [PubMed] [Google Scholar]
- Bindon C. I., Hale G., Brüggemann M., Waldmann H. Human monoclonal IgG isotypes differ in complement activating function at the level of C4 as well as C1q. J Exp Med. 1988 Jul 1;168(1):127–142. doi: 10.1084/jem.168.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown T. A., Mestecky J. Immunoglobulin A subclass distribution of naturally occurring salivary antibodies to microbial antigens. Infect Immun. 1985 Aug;49(2):459–462. doi: 10.1128/iai.49.2.459-462.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burton D. R., Gregory L., Jefferis R. Aspects of the molecular structure of IgG subclasses. Monogr Allergy. 1986;19:7–35. [PubMed] [Google Scholar]
- Childers N. K., Bruce M. G., McGhee J. R. Molecular mechanisms of immunoglobulin A defense. Annu Rev Microbiol. 1989;43:503–536. doi: 10.1146/annurev.mi.43.100189.002443. [DOI] [PubMed] [Google Scholar]
- Crago S. S., Kutteh W. H., Moro I., Allansmith M. R., Radl J., Haaijman J. J., Mestecky J. Distribution of IgA1-, IgA2-, and J chain-containing cells in human tissues. J Immunol. 1984 Jan;132(1):16–18. [PubMed] [Google Scholar]
- Ebersole J. L., Taubman M. A., Smith D. J., Frey D. E. Human immune responses to oral microorganisms: patterns of systemic antibody levels to Bacteroides species. Infect Immun. 1986 Feb;51(2):507–513. doi: 10.1128/iai.51.2.507-513.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ebersole J. L., Taubman M. A., Smith D. J., Genco R. J., Frey D. E. Human immune responses to oral micro-organisms. I. Association of localized juvenile periodontitis (LJP) with serum antibody responses to Actinobacillus actinomycetemcomitans. Clin Exp Immunol. 1982 Jan;47(1):43–52. [PMC free article] [PubMed] [Google Scholar]
- Ebersole J. L., Taubman M. A., Smith D. J., Socransky S. S. Humoral immune responses and diagnosis of human periodontal disease. J Periodontal Res. 1982 Sep;17(5):478–480. doi: 10.1111/j.1600-0765.1982.tb02032.x. [DOI] [PubMed] [Google Scholar]
- Farida R., Marsh P. D., Newman H. N., Rule D. C., Ivanyi L. Serological investigation of various forms of inflammatory periodontitis. J Periodontal Res. 1986 Jul;21(4):365–374. doi: 10.1111/j.1600-0765.1986.tb01470.x. [DOI] [PubMed] [Google Scholar]
- Flanagan J. G., Rabbitts T. H. Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing gamma, epsilon and alpha genes. Nature. 1982 Dec 23;300(5894):709–713. doi: 10.1038/300709a0. [DOI] [PubMed] [Google Scholar]
- Genco R. J., Zambon J. J., Murray P. A. Serum and gingival fluid antibodies as adjuncts in the diagnosis of Actinobacillus actinomycetemcomitans-associated periodontal disease. J Periodontol. 1985 Nov;56(11 Suppl):41–50. doi: 10.1902/jop.1985.56.11s.41. [DOI] [PubMed] [Google Scholar]
- Gray B. M. ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes. J Immunol Methods. 1979;28(1-2):187–192. doi: 10.1016/0022-1759(79)90340-5. [DOI] [PubMed] [Google Scholar]
- Hammarström L., Smith C. I. IgG subclasses in bacterial infections. Monogr Allergy. 1986;19:122–133. [PubMed] [Google Scholar]
- Koga T., Nishihara T., Fujiwara T., Nisizawa T., Okahashi N., Noguchi T., Hamada S. Biochemical and immunobiological properties of lipopolysaccharide (LPS) from Bacteroides gingivalis and comparison with LPS from Escherichia coli. Infect Immun. 1985 Mar;47(3):638–647. doi: 10.1128/iai.47.3.638-647.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loesche W. J., Syed S. A. Bacteriology of human experimental gingivitis: effect of plaque and gingivitis score. Infect Immun. 1978 Sep;21(3):830–839. doi: 10.1128/iai.21.3.830-839.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merrett J., Burr M. L., Merrett T. G. A community survey of IgG4 antibody levels. Clin Allergy. 1983 Sep;13(5):397–407. doi: 10.1111/j.1365-2222.1983.tb02615.x. [DOI] [PubMed] [Google Scholar]
- Mestecky J., Kilian M. Immunoglobulin A (IgA). Methods Enzymol. 1985;116:37–75. doi: 10.1016/s0076-6879(85)16005-2. [DOI] [PubMed] [Google Scholar]
- Mestecky J., Russell M. W. IgA subclasses. Monogr Allergy. 1986;19:277–301. [PubMed] [Google Scholar]
- Naito Y., Okuda K., Takazoe I. Detection of specific antibody in adult human periodontitis sera to surface antigens of Bacteroides gingivalis. Infect Immun. 1987 Mar;55(3):832–834. doi: 10.1128/iai.55.3.832-834.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naito Y., Okuda K., Takazoe I. Immunoglobulin G response to subgingival gram-negative bacteria in human subjects. Infect Immun. 1984 Jul;45(1):47–51. doi: 10.1128/iai.45.1.47-51.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ogawa T., McGhee M. L., Moldoveanu Z., Hamada S., Mestecky J., McGhee J. R., Kiyono H. Bacteroides-specific IgG and IgA subclass antibody-secreting cells isolated from chronically inflamed gingival tissues. Clin Exp Immunol. 1989 Apr;76(1):103–110. [PMC free article] [PubMed] [Google Scholar]
- Ogawa T., Tarkowski A., McGhee M. L., Moldoveanu Z., Mestecky J., Hirsch H. Z., Koopman W. J., Hamada S., McGhee J. R., Kiyono H. Analysis of human IgG and IgA subclass antibody-secreting cells from localized chronic inflammatory tissue. J Immunol. 1989 Feb 15;142(4):1150–1158. [PubMed] [Google Scholar]
- Page R. C. Gingivitis. J Clin Periodontol. 1986 May;13(5):345–359. doi: 10.1111/j.1600-051x.1986.tb01471.x. [DOI] [PubMed] [Google Scholar]
- Ranney R. R., Yanni N. R., Burmeister J. A., Tew J. G. Relationship between attachment loss and precipitating serum antibody to Actinobacillus actinomycetemcomitans in adolescents and young adults having severe periodontal destruction. J Periodontol. 1982 Jan;53(1):1–7. doi: 10.1902/jop.1982.53.1.1. [DOI] [PubMed] [Google Scholar]
- Rodbard D., Bridson W., Rayford P. L. Rapid calculation of radioimmunoassay results. J Lab Clin Med. 1969 Nov;74(5):770–781. [PubMed] [Google Scholar]
- Shakib F., Brown H. M., Stanworth D. R. Relevance of milk- and egg-specific IgG4 in atopic eczema. Int Arch Allergy Appl Immunol. 1984;75(2):107–112. doi: 10.1159/000233599. [DOI] [PubMed] [Google Scholar]
- Slots J., Listgarten M. A. Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases. J Clin Periodontol. 1988 Feb;15(2):85–93. doi: 10.1111/j.1600-051x.1988.tb00999.x. [DOI] [PubMed] [Google Scholar]
- Smith D. J., Gadalla L. M., Ebersole J. L., Taubman M. A. Gingival crevicular fluid antibody to oral microorganisms. III. Association of gingival homogenate and gingival crevicular fluid antibody levels. J Periodontal Res. 1985 Jul;20(4):357–367. doi: 10.1111/j.1600-0765.1985.tb00447.x. [DOI] [PubMed] [Google Scholar]
- Unkeless J. C., Scigliano E., Freedman V. H. Structure and function of human and murine receptors for IgG. Annu Rev Immunol. 1988;6:251–281. doi: 10.1146/annurev.iy.06.040188.001343. [DOI] [PubMed] [Google Scholar]
- Vincent J. W., Suzuki J. B., Falkler W. A., Jr, Cornett W. C. Reaction of human sera from juvenile periodontitis, rapidly progressive periodontitis, and adult periodontitis patients with selected periodontopathogens. J Periodontol. 1985 Aug;56(8):464–469. doi: 10.1902/jop.1985.56.8.464. [DOI] [PubMed] [Google Scholar]