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The cytotoxic effect of RTX+ and RTX− strains on HEp-2 cells in culture. Subconfluent HEp-2 monolayers were exposed for 1 hr to PBS-washed V. cholerae cells at a multiplicity of infection (MOI) of 100. The V. cholerae strains added were mock (PBS) (A), N16961 (B), WLV12 (C), and JAS6 (D).
