Table 1.
Assay for cytotoxic activity of V. cholerae strains
| Strain | Source (biotype)/Genotype | RTX | CTX | TLC | Toxicity |
|---|---|---|---|---|---|
| N16961 | Bangladesh patient, 1975 (E) | + | + | + | + |
| KFV42 | N16961 Δhap | + | + | + | + |
| P27459 | Bangladesh patient, 1976 (E) | + | + | + | + |
| E7946 | Bahrain patient, 1978 (E) | + | + | + | + |
| C6709 | Peru patient, 1991 (E) | + | + | + | + |
| MO10 | India patient, 1992 | + | + | + | + |
| Bang-1 | P27459 Δcore | + | − | + | + |
| Bah-1 | E7946 Δcore | + | − | + | + |
| Peru-1 | C6709 Δcore | + | − | + | + |
| Bengal-1 | MO10 Δcore | + | − | + | + |
| Bang-2 | P27459 ΔattRS1 | − | − | + | − |
| Bah-2 | E7946 ΔattRS1 | − | − | + | − |
| Peru-2 | C6709 ΔattRS1 | − | − | + | − |
| Bengal-2 | MO10 ΔattRS1 | − | − | + | − |
| WLV12 | N16961 rtxA∷pWL127 | − | + | + | − |
| WLV13 | P27459 rtxA∷pWL127 | − | + | + | − |
| WLV14 | E7946 rtxA∷pWL127 | − | + | + | − |
| WLV15 | C6709 rtxA∷pWL127 | − | + | + | − |
| WLV16 | MO10 rtxA∷pWL127 | − | + | + | − |
| JAS6 | N16961 rtxC∷pJAS6 | − | + | + | − |
| O395 | India patient, 1964 (C) | − | + | + | − |
| 569B | India patient, 1948 (C) | − | + | + | − |
The V. cholerae biotype is listed in parenthesis: classical (C) or El Tor (E). MO10 is a V. cholerae 0139 strain. Δcore is a deletion of the CTX element except the RS element (45). ΔattRS1 is a deletion involving the two HindIII sites bracketing the CTX element (10, 12, 16). Southern analysis of DNA prepared from the indicated strains or PCR with approporiate primers was used to detect rtxA or rtxC, ctxAB, and the TLC element. Cytotoxicity was scored visually in a blind assay.