Figure 1.

Computer generated histograms from 3 different images after staining with EB and Syto 13. The red line characterizes the relative pixel intensity of the red pixels. The green line characterizes the relative pixel intensity of the green pixels. The vertical red and green dotted lines indicate the threshold determined by the empirical 85% pixel cutoff determined by the cumulative histograms seen in Figure 2. "# pixels" indicates number of pixels.