Figure 3.

Relationship between number of repeats in the hmw2A promoter, quantity of HMW2 produced, and the level of hmw2A transcript. In the blots in A and B, lanes were loaded as labeled with either 12Δhmw1p variants containing 15 and 17 repeats or 12.234Δhmw1p variants containing 23, 26, and 28 repeats. (A) Variants were analyzed for levels of HMW2 by Western blot by using the polyclonal antiserum 28G. (B) Variants were analyzed for the level of hmw2A mRNA by Northern blot, probing with a specific internal fragment of hmw2A. The resulting signals were quantitated by a PhosphorImager, and the hmw2A transcript levels were corrected for slight differences in loading based on the porA transcript, which is expressed constitutively. The percentages were determined by setting the isolate with 15 repeats at 100% and comparing the relative intensity of each subsequent isolate.