Table 1.
Specific and per g enzyme activities in brown trout liver
| Enzymes |
Activity per g of liver s-1 g-1 liver (*) or nmol min-1 g-1 liver |
Specific activity s-1 mg-1 protein (*) or nmol min-1 mg-1 protein |
||||
| A | B | D | E | F | ||
| Peroxisomal markers | ||||||
| Catalase (*) | 95.9 (0.4) | 1.3 (0.2) | 2.0 (0.3) | 3.5 (0.3) | 0.4 (0.3) | 0.6 (0.2) |
| Urate oxidase | 790.4 (0.6) | 10.3 (0.6) | 13.0 (0.7) | 22.6 (0.6) | 4.7 (0.8) | 8.8 (0.8) |
| D-aminoacid oxidase | 471.0 (0.6) | 6.0 (0.4) | 8.9 (0.5) | 14.4 (0.1) | 1.4 (0.4) | 3.5 (0.4) |
| Fatty acyl-CoA oxidase | 73.3 (0.5) | 1.0 (0.4) | 0.4 (0.5) | 1.5 (0.3) | 0.3 (0.8) | 1.0 (0.5) |
| Glycolate oxidase | 60.2 (0.5) | 0.8 (0.3) | 0.8 (0.4) | 2.3 (0.4) | 0.5 (0.5) | 0.7 (0.4) |
| Mitochondrial marker | ||||||
| Succinate dehydrogenase | 310.7 (0.5) | 3.6 (0.3) | 29.1 (0.5) | 27.8 (0.5) | 1.4 (0.7) | 0.2 (0.6) |
| Lysosomal marker | ||||||
| Aryl sulphatase | 275.0 (0.2) | 3.2 (0.4) | 10.4 (0.5) | 10.6 (0.5) | 2.4 (0.4) | 1.4 (0.4) |
| Microsomal marker | ||||||
| NADPH cytochrome c reductase | 2520 (0.4) | 32.5 (0.3) | 29.2 (0.4) | 41.8 (0.3) | 99.0 (0.5) | 11.5 (0.3) |
|
Protein per g of liver (mg g-1 liver) |
Total protein per fraction (mg) |
|||||
| 77.8 (0.2) | 225.6 (0.2) | 26.4 (0.5) | 22.3 (0.3) | 25.4 (0.6) | 128.4 (0.2) | |
Activities per g of liver and specific activities were measured at 37°C in liver homogenates and cell fractions. Protein per mg of liver and in cell fractions is also given. A – liver homogenates; B – crude mitochondrial/lysosomal fraction; D – crude peroxisomal fraction; E – crude microsomal fraction; F – cytosolic fraction. Presented data are a mean from 6 fishes and the coefficient of variation is given in brackets. (*) – Catalase activity is expressed by the first-order rate constant (s-1) and all other enzyme activities are express in nmol min-1, per mg of protein or per g of liver.