Table 1.
Specific interference with virus infection by bacterial crude preparations
Bacterial preparation | Phenotype | Virus titer |
Coinoculation (PPV)* | ||
HT115 | S 3/3 | 1.221 ± 0.105 |
PPV HC dsRNA | R 14/17 | 0.007 ± 0.004 |
S 3/17 | 1.197 ± 0.060 | |
PPV CP dsRNA | R 8/11 | 0.017 ± 0.017 |
S 3/11 | 1.310 ± 0.103 | |
PMMoV IR 54 | S 7/7 | 1.149 ± 0.078 |
Non-inoculated controls | 0.012 ± 0.007 | |
Spraying (PMMoV)† | ||
PMMoV IR 54 (0 day) | R 5/5 | 0.004 ± 0.002 |
PMMoV IR 54 (1 day) | R 5/5 | 0.009 ± 0.003 |
PMMoV IR 54 (3 days) | R 5/5 | 0.011 ± 0.010 |
PMMoV IR 54 (5 days) | R 18/18 | 0.015 ± 0.012 |
PMMoV IR 54 (7 days) | R 1/5 | 0.012 |
S 4/5 | 1.360 ± 0.141 | |
HT115 (5 days) | S 10/10 | 1.420 ± 0.121 |
Non-inoculated controls | 0.010 ± 0.009 | |
Spraying (PPV)‡ | ||
HT115 | S 7/7 | 1.042 ± 0.260 |
PPV HC dsRNA | R 11/12 | 0.004 ± 0.008 |
S 1/12 | 0.278 | |
Non-inoculated controls | 0.008 ± 0.003 |
*Plants were inoculated with mixtures of PPV plus French Press preparations derived from HT115 harboring either PPV HC dsRNA, PPV CP dsRNA, PMMoV IR 54 or the empty vector (HT115). The number of plants showing resistant (R) or susceptible (S) phenotypes to viral infection is indicated. Virus titer corresponds to the mean ELISA values ± SE at 12 dpi. †PMMoV was inoculated on plants simultaneously or one, three, five or seven days after spraying with either PMMoV IR 54 or HT115 preparations in the same leaves. ‡PPV was inoculated on plants five days after spraying with either PPV HC dsRNA or HT115 preparations in the same leaves.