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. 2003 Apr 1;100(8):4969. doi: 10.1073/pnas.0930700100

Figure 1.

Figure 1

Cell sorting and flow cytometric analysis of MeWo-FTIII cells. (A) MeWo-FTIII cells were stained with anti-sialyl Lewis x antibody (CSLEX-1) then by FITC-conjugated secondary antibody, and sorted by FACStar. Cells indicated by open bars were pooled and designated as MeWo-FTIII·N (negative), MeWo-FTIII·M (moderate), and MeWo-FTIII·H (highly positive). (B–F) Cultured MeWo-FTIII·N (N), MeWo-FTIII·M (M), and MeWo-FTIII·H (H) cells were subjected to flow cytometry analysis after staining with anti-sialyl Lewis x (B), anti-sialyl Lewis a (C), or anti-Lewis x antibody (D), followed by FITC-conjugated secondary antibody or FITC-conjugated Sambucus nigra agglutinin (E) or FITC-conjugated tomato lectin (F). HL-60 cells also were stained in B.