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. 2003 Apr 7;100(8):4429–4434. doi: 10.1073/pnas.0730832100

Figure 2.

Figure 2

Detection of β-glucuronidase in Gus and GusT-transformed N. benthamiana plants. Twenty micrograms of plant protein extracts were separated under nondenaturing conditions, and gels were stained for β-glucuronidase activity. Lanes 1, 8, and 10, protein extract of nontransformed (wild-type) plants; lanes 2 and 9, protein extract of wild-type plants supplemented with 100 ng of β-glucuronidase purified from E. coli; lanes 3–7, protein extracts from lines GusT 1.5, GusT 5.1, GusT 6.2, GusT 8.1, and Gus 5.a, respectively.